Electron-nuclear double resonance study of flavodoxin from Peptostreptococcus elsdenii.
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چکیده
منابع مشابه
Dependence of the proton magnetic resonance spectra on the oxidation state of flavodoxin from Clostridium MP and from Peptostreptococcus elsdenii.
The broadening of protein nuclear magnetic resonances in the spectra of the semiquinone forms of flavodoxins derived from Clostridium MP and Peptostreptococcus elsdenii relative to the resonances in the oxidized and reduced forms is highly selective. Spectra from both species of flavodoxin indicate that conformational differences between the oxidized and fully reduced states are minor and, cons...
متن کاملStudies on the Kinetics and Mechanism of Reduction of Flavodoxin
1 The reactions of the oxidised and semiquinone forms of Peptostreptococcus elsdenii flavodoxin with sodium dithionite have been studied by stopped flow spectrophotometry. At low ionic strength where comproportionation of flavodoxin is slow compared with the overall rate of reduction, the reactions with an excess of dithionite follow first-order kinetics. Semiquinone is not detected during the ...
متن کاملIdentification and properties of 8-hydroxyflavin--adenine dinucleotide in electron-transferring flavoprotein from Peptostreptococcus elsdenii.
1. A new flavin prosthetic group has been isolated in pure form from the electron-transferring flavoprotein of Peptostreptococcus elsdenni. Its structure has been established as the FAD derivative of 7-methyl-8-hydroxyisoalloxazine: (see article). Proof of this structure has been obtained by chemical syntehsis of 7-methyl-8-hydroxyisoalloxazine models, and by stepwise degradation of the native ...
متن کاملEvidence for a Novel Flavin Prosthetic Group Associated with Nadh Dehydrogenase From
I. NADH dehydrogenase (EC 1.6.99.3) has been purified from the strictly anaerobic rumen bacterium Peptostreptococcus elsdenii. The purified protein is specific for NADH as electron donor; it can use 2,6-dichlorophenolindophenol (DCIP), cytochrome c, KzFe(CN)6 or flavodoxin as electron acceptor. It has a molecular weight of about 63 ooo. The absorption spectrum shows maxima at 475, 375, 356, 318...
متن کاملBba 66291 Studies on Flavin Binding in Flavodoxins
I. Stable apoproteins have been prepared from Peptostreptococcus elsdenii, C. pasteurianum and Clostridium MP flavodoxins by dialysis of the native proteins against 2 M KBr at pH 3.9 and 3" lO-4 M EDTA. The apoproteins each bind I molecule of FMN to give complexes identical with the native flavodoxins. 2. Binding causes almost complete quenching of both protein and FMN fluorescence. This proper...
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عنوان ژورنال:
- Helvetica chimica acta
دوره 56 7 شماره
صفحات -
تاریخ انتشار 1973